Dissemination of blaOXA-23 in Acinetobacter spp. in China: main roles of conjugative plasmid pAZJ221 and transposon Tn2009.
نویسندگان
چکیده
Production of the OXA-23 carbapenemase is the most common reason for the increasing carbapenem resistance in Acinetobacter spp. This study was conducted to reveal the genetic basis of blaOXA-23 dissemination in Acinetobacter spp. in China. A total of 63 carbapenem-resistant OXA-23-producing Acinetobacter sp. isolates, representing different backgrounds, were selected from 28 hospitals in 18 provinces for this study. Generally, two patterns of plasmids carrying blaOXA-23 were detected according to S1-nuclease pulsed-field gel electrophoresis and Southern blot hybridization. A ca. 78-kb plasmid, designated pAZJ221, was found in 23 Acinetobacter baumannii and three Acinetobacter nosocomialis isolates, while a novel ca. 50-kb plasmid was carried by only two other A. baumannii isolates. Three of these isolates had an additional copy of blaOXA-23 on the chromosome. Transformation of the two plasmids succeeded, but only pAZJ221 was conjugative. Plasmid pAZJ221 was sequenced completely and found to carry no previously known resistance genes except blaOXA-23. The blaOXA-23 gene of the remaining 35 isolates was chromosome borne. The blaOXA-23 genetic environments were correlated with Tn2009 in 57 isolates, Tn2008 in 5 isolates, and Tn2006 in 1 isolate. The MIC values for the carbapenems with these isolates were not significantly associated with the genomic locations or the copy numbers of blaOXA-23. Overall, these observations suggest that the plasmid pAZJ221 and Tn2009 have effectively contributed to the wide dissemination of blaOXA-23 in Acinetobacter spp. in China and that horizontal gene transfer may play an important role in dissemination of the blaOXA-23 gene.
منابع مشابه
Dissemination of bla OXA - 23 in Acinetobacter spp . in China : main roles of 1 conjugative plasmid pAZJ 221 and transposon Tn 2009
15 Production of OXA-23 carbapenemase is the most common reason for the increasing 16 carbapenem resistance in Acinetobacter spp. This study was conducted to reveal the genetic 17 basis of the blaOXA-23 dissemination in Acinetobacter spp. in China. A total of sixty-three 18 carbapenem-resistant OXA-23-producing Acinetobacter spp. isolates, representing different 19 backgrounds, were selected fr...
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 59 4 شماره
صفحات -
تاریخ انتشار 2015